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PluraPath™ FAQS

Frequently Asked Questions

 

Can you determine whether the pathogen being tested is alive or dead in the water sample?

No, PluraPath™ does not distinguish viable from non-viable targeted DNA.


What is the Limit of Detection?

PluraPath™ is capable of testing as low as 1 CFU/mL.


Would Nephros provide a service using this device for our company needs?

Nephros currently has the capability to deploy a rapid response team to address any in extremis pathogen screening or detection needs you may have.


What training is involved for becoming proficient using PluraPath™? Continuing training?

Nephros offers a distance training option through our web portal, or, a one-day onsite instruction followed by two field-training days. Web-conferencing for refresher training can be scheduled as needed through our website here. 


How does the Multistrip differ from the Monostrip? 

Multistrips allow you to screen for up to 15 different pathogens in one water sample at the same time. Monostrips allow you to screen eight (8) water samples for one specific pathogen, all at the same time. 


How do you recommend integrating PluraPath™ into our water risk management plan?

Nephros recommends using PluraPath™ as a screening, baselining, or validation tool in concert with traditional lab-based testing. The mobile and rapid deployment of PluraPath™ allows you to have early and reliable indicators of pathogen concentrations in water samples.


How often does the qPCR machine need to be calibrated?

The manufacturer recommends calibration once per year or in situations where the lid height has been adjusted. 


Is the qPCR machine manufactured by Nephros?

No, Chai Bioscience manufactures the qPCR machine.


How small is the pore size of the FilPath™ filter?

5nm pore. 0.005 micron.


What certifications are required to operate the PluraPath™ system?

Nephros will supply distance learning or onsite training to certify you on PluraPath™.


What do the consumables include and what is the cost?

PluraPath™ consumables include the lysis buffer, FilPath™ filter, 50mL conical tube, 20mL syringe, disposable bleach wipe, 1L whirl-pak bag, and the Monostrip and Multistrip strips. For cost information, please contact christian@nephros.com.


Why did you choose the list of pathogens currently being tested on the multistrips?

The current list of pathogens originates from a list of waterborne pathogens highlighted by the CDC.


How do reports of the results from the qPCR get populated and how should I interpret the results?

Reports can be populated using our custom Nephros spreadsheet or our Nephros mobile app (suitable for Apple and Android platforms).


Is Wi-Fi necessary to operate the equipment?

A Wi-Fi connection, mobile hot-spot, or ethernet cable is needed to connect your Chai qPCR machine to the internet. A laptop, tablet, or phone can be used to run the experiment once an account is established. For detailed instructions on network connection and graphic user interface of your qPCR machine, contact chrisitan@nephros.com.


What is the shelf-life of the consumables; reagents/strips; lysis buffer?

Consumable packs have a 12-month shelf-life if stored at ambient temperatures. Monostrip and Multistrip reagents have a 6-month shelf-life if stored at room temperature. 12-months or more if refrigerated. Lysis buffer has a 12-month shelf-life when stored at ambient temperatures.


Who services the qPCR machine if it breaks?

Nephros PDS staff will provide any immediate assistance. If a resolution cannot be made Chai bioscience technical support will render services. Contact christian@nephros.com to schedule services with Nephros PDS.


How long does it take to complete one test?

It takes approximately 1 hour to complete a multistrip test. It takes approximately 1 hour 30 minutes to complete a mono strip test.


What regulations exist currently that require we test the pathogens you have listed?

Currently, there are no regulations that require testing of the pathogens we have listed other than CMS and ASHRAE guidance on Legionella.


How do you test water that has qPCR inhibitors present?

Nephros has a proprietary wash that can be used address any PCR inhibitors present in your water sample. For cost and purchasing options for our PIM Kits, contact sales@nephros.com.


Could we ship a sample of water for you to test for us? What might that service look like?

Nephros is happy to test any water samples you might have. For options that fit your needs, contact christian@nephros.com.


Can the FilPath™ filters be used more than once; or, is it single use?

The filters are single use only. Repeat use of filters will provide unreliable results.


What measures are taken to prevent contamination during testing?

Nephros recommends adhering to ISO 12869_2019 standards, which includes using a ​1:10 bleach solution or 70% ethanol solution before each testing cycle. 


Are you able to determine the remaining biological burden in my water after your tests?

Nephros can use SequaPath™ a patented technology that uses the 16S rRNA to determine any remaining bacterial biological burden in your water supply. 


Does PluraPath™ adhere to ISO standards for Legionella?

Nephros is currently working toward having an ISO 12869_2019 accreditation for PluraPath™.

 

Can’t Find it Here? Contact our Senior Manager of Pathogen Detection, Christian Railsback: christian@nephros.com